Promotionsvortrag Physik: „Single-particle cryogenic light microscopy“

Date: December 11, 2023Time: 3:30 pm – 5:00 pmLocation: Leuchs-Russell-Auditorium, MPI, Staudtstr. 2, Erlangen

Ankündigung des Promotionsvortrags von: Herrn Franz Ferdinand Wieser

Super-resolution optical microscopy elucidates cellular structure beyond the diffraction limit,

enabling discoveries in the life sciences. Cryogenic optical localization provides further depth,

exploiting the enhanced photo-stability of organic fluorophores at low temperatures and their

random on-off switching to determine the position of several labelling sites with Angstrom

precision. Therefore, the method advances optical microscopy to the three-dimensional molecular

architecture of proteins and bio-molecular assemblies.

In this talk, we start with the detection of single-molecules with an optical microscope, demonstrate

how we achieve super-resolution via precise localization, and go on to explore potential

applications. The polarization of the emitted fluorescence light is included as an additional

parameter in the identification and localization of several molecules within a diffraction-limited

area. An application for determining inter-molecular distances of a few nanometers is discussed

using DNA origami nanorulers. In another study of a small protein complex, the full potential for

determining three-dimensional structures through the application of single-particle analysis is

illustrated.

We also show that we can combine this approach with image classification to address

heterogeneous samples. Lastly, we present a workflow for the preparation and imaging of vitrified

specimens adapted to our optical microscope, allowing quantitative information on the structure

of macro-molecules in a near-native state and environment to be obtained. Exciting prospects are

correlative studies with cryogenic electron microscopy and tomography, particularly of membrane

proteins.

(Vortrag auf Englisch)

Dem Vortrag schließt sich eine Diskussion von 15 Minuten an. Vortrag und Diskussion sind öffentlich. Diesen Verfahrensteilen folgt ein nicht öffentliches Rigorosum von 45 Minuten.

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Event Details

Date:
December 11, 2023
Time:
3:30 pm – 5:00 pm
Location:

Leuchs-Russell-Auditorium, MPI, Staudtstr. 2, Erlangen

Event Categories:
Promotionen